Section

Ecology

Title

ON ECO-GENETIC MONITORING OF THE POPULATIONS OF FINE-LEAVED PEONY (PAEONIA TENUIFOLIA L.), A FORAGE PLANT OF THE PELATEA KLUGIANA (FREYER, 1836) (LEPIDOPTERA, TORTRICIDAE), A RARE AND ENDEMIC SPECIES OF LEPIDOPTERA IN THE ULYANOVSK REGION (THE MIDDLE VOLGA REGION)

Сontributors

A. V. Mishchenko, Associate Professor, Ph. D. (Biology), This email address is being protected from spambots. You need JavaScript enabled to view it.,

E.A. Artemieva, Professor, Ph. D. (Biology), Dr. Habil, This email address is being protected from spambots. You need JavaScript enabled to view it.,

D.K. Makarov, postgraduate student, This email address is being protected from spambots. You need JavaScript enabled to view it.,

V. V. Soltis, Head of the organization of research and patent-licensed software, This email address is being protected from spambots. You need JavaScript enabled to view it..

Ulyanovsk State Pedagogical University of I. N. Ulyanov

Abstract

Biomonitoring and molecular genetic studies of populations of a rare and endangered species of insects Pelatea klugiana, and fine-leaved forage plant of peony Paeonia tenuifolia, listed in the Red Data Book of the Russian Federation and the Ulyanovsk Region, were held during the field seasons of 2009—2016. The seeds' surface was studied for the presence of fine-leaved peony pathogens, as a result four kinds of bacteria were detected by molecular cloning. The number of local populations of peony bud worms P. klugiana reaches 15—25 nests per 1 hectare. The peony moth is monophagous, it feeds exclusively on leaves, flowers and buds of Paeonia tenuifolia, the less closely related species are Paeonia tenuifolia, P. biebersteiniana, which form a compact nest on the tops of braided cob-webs of the generative shoots of the plant. These species of plants are also rare and endangered and are listed in the Red Data Book of the Russian Federation and the Ulyanovsk Region. As a result of molecular-genetic studies of the surface of fine-leaved peony seeds, i.e. the food plant P. klugiana, 4 types of bacteria are revealed, including only one pathogenic species for plants: Agrobacterium tumefaciens (Rhizobium radiobacter), phytopathogens that can cause the formation of crown galls; Bacillus cereus; Bacillus pumilus; Pseudomonas fluorescens. The importance of such fundamental issues as the screening of the presence of pathogens in the cultures and specimens of rare species of plants, stems from the need to monitor the state of host plants of rare species of insects, which can be exposed to stress and unfavorable factors of plant pathogens invasion. In the course of the work, an attempt to develop laboratory protocols was made to allow, in the presence of equipment for molecular genetic studies, to identify phytopathogens on the surface of the rare plant seeds, rare prey insect species listed in the Red Data Book of the Russian Federation and the Ulyanovsk Region.

Keywords

biomonitoring, bacteria, molecular cloning, Lepidoptera, the Red Data Book, the Middle Volga Region.

References

1.  Nedoshivina S. V. 2006. Materialy po faune listovertok Ulyanovskoy oblasti (Lepidoptera, Tortricidae). Novye dannye i dopolneniya po vidovomu sostavu // Priroda Simbirskogo Povolzhya. [Materials on the fauna of Tortricidae of the Ulyanovsk Region (Lepidoptera, Tortricidae). New data and additions to species composition. The Nature of the Simbirsk Volga Region]. Sb. nauch. tr. [Bul. scientific. Papers.]. Vol. 7. Ulyanovsk: Izd-vo “Korporatsiya tekhnology prod- vizheniya”. P. 140—148. (in Russian)

2.  Anikin V. V., Sachkov S. A., Zolotuhin V. V., Nedoshivina S. V., Trofimova T. A. 2006. “Fauna lepidopterologica Volgo- Uralensis” 150 years later: changes and additions. Part 9. Tortricidae (Insecta, Lepidoptera) Atalanta, 2006.

3.  Nedoshivina S. V., Zolotuhin V. V. 2005. A new subspecies of Pelatea klugiana (Freyer, 1836) from the Middle Volga Region of Russia with notes on its morphology and biology (Tortricidae) Nota lepid. No. 28 (1). P. 31—37.

4.  Life Technologies Corporation URL: http://www.lifetechnologies.com. Электронный ресурс (дата обращения 2.12.2016 г.).

5.  Artemyeva E. A., Kovalev A. V., Krason B. A. Redkiye nasekomye klyuchevykh territory Ulyanovskoy oblasti. Priroda Simbirskogo Povolzhya. [Rare insect key areas of the Ulyanovsk region. Nature of the Simbirsk Volga region]. Sb. nauch. tr. [Bull. of scientific. papers.]. Vol. 11. Ulyanovsk: Izd-vo “Korporatsiya tekhnology prodvizheniya”, 2010. P. 107—113. (in Russian)

5.  GenBank URL: http://www.ncbi.nlm.nih.gov/genbank. Электронный ресурс (дата обращения 12.12.2016 г.).

6.  Artemyeva E. A., Krason B. A., Kovalev A. V. Redkiye vidy nasekomykh v klyuchevykh i novykh perspektivnykh OOPT Ulyanovskoy oblasti // Priroda Simbirskogo Povolzhya. [Rare species of insects in key and promising new protected areas of Ulyanovsk region. Nature of the Simbirsk Volga region.] Sb. nauch. Tr. [Bul. scientific. Papers]. Vol. 10. 2009. Ulyanovsk: Publishing house “Corporation of promotion technologies”. P. 138—144. (in Russian)

7.  Artemyeva E. A., Korolkov M. A., Semenov D. Yu. Materialy po krasnoknizhnym vidam izbrannykh grupp zhivotnykh fauny Ulyanovskoy oblasti. Monografiya [Materials for red data book species of selected groups of animals of the fauna of the Ulyanovsk Region]. Monograph. Ulyanovsk: Izd-vo “Korporatsiya tekhnology prodvizheniya”. 2013. 88 p. (in Russian)

8.  Krasnaya kniga Ulyanovskoy oblasti [The red book of the Ulyanovsk Region]. 2015. Moscow, Publishing “Buki Vedi”, 550 p. (in Russian)

9.  Hulse M., Johnson S., Ferrieri P. 1993. Agrobacterium infections in humans: experience at one hospital and review Clinical Infectious Diseases. Vol. 16. P. 112—117.

10. Kotiranta A., Lounatmaa K., Haapasalo M. 2000. Epidemiology and pathogenesis of Bacillus cereus infections Microbes and Infection. Vol. 2. No. 2. P. 189—198.

11. Akhtar M. S., Shakeel U., Siddiqui Z. A. 2010. Biocontrol of Fusarium wilt by Bacillus pumilus, Pseudomonas alcaligenes and Rhizobium sp. on lentil. Archives of Phytopathology and Plant Protection. Vol. 43. P. 1423—1434.

12. Haas D., Defago G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads Nature Reviews Micro-biology. Vol. 3. P. 307—319.