DOI: 10.24411/1728-323X-2018-12060

Section

Ecology

Title

PRIMER SYSTEMS USED TO IDENTIFY REPRESENTATIVES OF THE COMMUNITY OF CHEMOLITHOTROPHIC MICROORGANISMS IN THE SHANUCH DEPOSIT (KAMCHATKA)

Сontributors

S.        V. Rogatykh, Senior Researcher, This email address is being protected from spambots. You need JavaScript enabled to view it., Research Geotechnological Centre,

Far Eastern Branch of Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russia.

Abstract

This paper presents the experimental data obtained in the development of test systems based on the polymerase chain reaction necessary for a qualitative and quantitative analysis of the structure of the communities of chemilithotrophic acidophilic microorganisms Acidithiobacillusthiooxidans, Acidi- thiobacillusferrooxidans, Sulfobacillusthermosulfidooxidans and Ferroplasmaacidiphillum isolated from sulfide ores of copper-nickel deposit Shanuch (Kamchatka). On the basis of the sequences obtained for the samples of A. thiooxidans and A. ferrooxidans, oligonucleotide systems with species specificity were created, which were subsequently used in the genotyping reaction. To develop primers S. thermosulfidooxidans and

F.         acidiphillum, sequences of the GenBank database were used. The alignment of the sequences obtained revealed the variable regions in the genomes of A. thiooxidans and A. ferrooxidans and subsequently used them when writing species-specific oligonucleotides. The specificity of the primers used in the study was checked by sequencing. The sequences of all the PCR products obtained corresponded to the sequences obtained for the clone collection.

Keywords

bioleaching, oligonucleotides, acidophilic microorganisms, microbial community, copper-nickel deposit.

References

1.  Bulaev A. G., Pershina E. V., Ukraincev I. V. The state of development of modern biohydrometallurgical technologies and the prospects for their use in Russia // Non-ferrous metals. 2016. No. 10. P. 29—35. [in Russian]

2.  Hajnasova T. S., Levenec O. O. Bacterial-chemical leaching as an environmentally safe method for processing sulphide cobalt- copper-nickel ore // Exploration and protection of mineral resources. 2015. No. 1. P. 49—54. [in Russian]

3.  Kondrat'eva T. F., Bulaev A. G., Murav'ev M. I. Microorganisms in biogeotechnologies for processing sulphide ores. Moscow, Nauka, 2015. 206 p. [in Russian]

4.  Sovmen V. K., Gus'kov V. N., Belyj A. V. Processing of gold-bearing ores with application of bacterial oxidation in conditions of the Far North. Novosibirsk, Nauka, 2007. 144 p. [in Russian]

5.  Rogatykh S. V., Dokshukina A. A., Levenets O. O., Muradov S. V., Kofiadi I. A. Evaluation of Quantitative and Qualitative Composition of Cultivated Acidophilic Microorganisms by Real-Time PCR and Clone Library Analysis // Microbiology.

2013.   Vol. 82. No. 2. P. 210—214. [in Russian]

6.  Zaulochnyj P. A. Intensifikacijatehnologiibakterial'nogovyshhelachivanijaupornyhzolotosul'fidnyhkoncentratov s is- pol'zovaniemassociaciimikroorganizmov, vkljuchajaumerenno-termofil'nyebakterii: dis.... kand. tehn. nauk: 25.00.13. Mos-cow, 2011. 168 p. [in Russian]

7.  Aswegen P. C. van, Niekerk J. van, Olivier W. The BIOX™ process for the treatment of refractory gold concentrate // In: Biomining. Ed. by D. E. Rawlings and D. Barrie Johnson. Springer—Verlag Berlin Heidelberg, 2007. P. 1—35.

8.  Foucher S., Battaglia-Brunet F., d’Hugues P., Clarens M., Godon J. J., Morin D. Evolution of the bacterial population during the batch bioleaching of a cobaltiferous pyrite in a suspended-solids bubble column and comparison with a mechanically agitated reactor // Hydrometallurgy. 2003. No. 71. P. 5—12.

9.  Harvey T. J., Bath M. The GeoBiotics GEOCOAT® Technology — progress and challenges // In: Biomining. Ed. By

D.  E. Rawlings and D. Barrie Johnson. Springer—Verlag Berlin Heidelberg, 2007. P. 97—112.

10.       Puhakka J. A., Kaksonen A. H., Riekkola-Vanhanen M. Heap Leaching of Black Schist // In: Biomining. Ed. By D. E. Rawl-ings and D. Barrie Johnson. Springer—Verlag Berlin Heidelberg, 2007. P. 139—152.